文献简介

出版社:International Journal of Surgical Pathology

作  者:Tomonori Hikita, MD1, Yuji Ohtsuki, MD2, Toshiharu Maeda, MD3, and Mutsuo Furihata, MD1

编  号:

关键字:extramammary Paget’s disease, immunohistochemistry, fluorescence in situ hybridization (FISH), CK19, HER-2

年  份:2012   点击量:845

文献摘要

To determine useful immunohistochemical markers for tumor cells in extramammary Paget’s disease (EMPD), immunohistochemical (IHC) examinations in 17 patients with EMPD, including 4 patients with dermal invasion, were performed. Among the antibodies examined, cytokeratin 7 (CK7) and CK19 were strongly positive for both intraepidermal and dermally invasive tumor cells in all patients. CAM5.2 and mucin 1 (MUC1) were also good markers. Although IHC examination revealed positive for HER-2 in 4 EMPD patients with dermal invasion, 4 out of 13 noninvasive patients were IHC negative. Fluorescence in situ hybridization (FISH) study revealed negative results for HER-2 gene amplification in 8 IHC positive patients. Our results show that besides CK7, CK19 is another favorable marker of tumor cells of EMPD. Four patients with dermal invasion were strongly positive for HER-2, although negative results were obtained in the FISH study. Further investigations are required to confirm the results of the FISH study.

为了确定乳房外Paget病(EMPD)中有效的肿瘤细胞免疫组化标记物,我们对17EMPD患者,其中包括4名真皮浸润的患者进行免疫组化(IHC)检查。在抗体检查中,所有患者的表皮内和经皮浸润性肿瘤细胞中,细胞角蛋白7CK7)和CK19呈强阳性。CAM5.2和黏蛋白1MUC1)也是很好的标志物。。尽管IHC检查提示在4名真皮浸润的EMPD患者中HER-2呈阳性,但其他的13名非浸润性患者IHC提示为阴性荧光原位杂交(FISH)研究表明在8IHC检查呈阳性的患者中,HER-2基因扩增显示阴性结果。我们的研究结果表明除CK7CK9之外的,EMPD肿瘤细胞另外一个有效的标志物。尽管在FISH研究中得出阴性的结果,但在4名真皮浸润患者中HER-2呈强阳性。。需要进一步的调查来确定FISH的研究结果。