文献简介

出版社:Journal of Dermatology

作  者:Zhang M ; Wu N ; Yang L ; Zhang J ; Sun X ; Zhong S ; Ma X ; Wang Y

编  号:10.1111/j.1346-8138.2011.01289.x

关键字:blister fluid, cell-mediated immunity, cytokine, cytometric bead array, herpes zoster.

年  份:2011   点击量:930

文献摘要

ABSTRACT

摘要

Herpes zoster (HZ) is a Varicella zoster virus infection disease. Previous studies have presumed the connection between development of HZ and involvement of cellular immunity in peripheral blood. However, whether cellular immunity plays a role in the local skin lesion has not been addressed. To explore the levels of T-helper cell Th1⁄Th2 type cytokine profiles in the blister fluid of the skin lesions from the patients with HZ and its role in pathogenesis, we used the cytometric bead array kit to compare the levels of cytokines (interleukin [IL]-2, tumor necrosis factor [TNF]- α, IL-10 and IL-4) in blister fluid from 46 patients with those from the suction blister fluids from 20 volunteers without any infectious disease (the control group). The results indicated that the levels of Th1 cytokinesIL-2 and TNF-a in the blister fluid from the patients’ skin lesions were significantly lower than those from the control group, whereas the levels of Th2 cytokines IL-10 and IL-4 were significantly higher than those in the control group. Moreover, significant variation of the levels of Th1⁄Th2 cytokines (IL-2, TNF-a, IL-10 and IL-4) in the blister fluid from the HZ patients’ lesions was also observed among different stages of the disease. It is concluded that a cytokine imbalance was present in the local lesions of patients with HZ during disease development. Our data suggested that the Th immunity was associated with disease activity, which may play an important role in the pathogenesis of HZ.

带状疱疹(HZ)是一种由水痘-带状疱疹病毒感染引起的疾病。以往的研究认为HZ的发展与外周血细胞免疫的参与之间存在联系。然而,细胞免疫在局部皮肤损伤中是否发挥作用这一问题还尚未解决。为探索带状疱疹患者皮损处水泡液中辅助性T细胞Th1/Th2型细胞因子水平及其在发病机制中发挥的作用,我们使用流式微珠阵列盒对来自46例患者水泡液中以及20名无任何传染病的志愿者(对照组)负压吸疱液中的细胞因子(白介素[IL]-2,肿瘤坏死因子[TNF]-α,IL-10IL-4)水平进行了比较。研究结果表明,患者皮损处水泡液中的Th1型细胞因子IL-2TNF-α的水平显著低于对照组,但是Th2型细胞因子IL-10IL-4的水平却显著高于对照组。此外,我们也观察到在疾病的不同阶段HZ患者损伤处泡液中Th1⁄Th2型细胞因子(IL-2, TNF-a, IL-10IL-4))水平存在显著差异。从而得出结论,在HZ病情发展过程中,患者的局部病变组织存在细胞因子失衡。我们的数据表明,辅助T细胞免疫与疾病活动相关,并且可能在HZ的发病机制中发挥重要作用。

 

Zhang M et.al ; Journal of Dermatology ; 2011 Dec; Vol. 38 (12), pp