文献简介

出版社:Elsevier Inc.

作  者:Cecilia G. Clement MD, Luan D. Truong MD

编  号:10.1016/j.humpath.2014.04.016

关键字:amyloidosis

年  份:2014   点击量:1229

文献摘要


Summary Congo red stain apple-green birefringence under polarized light is the most popular method for detecting amyloid; however, it has limitations. The goal of this study was to evaluate if examination of Congo red stain by fluorescent microscopy (FM) significantly enhances the diagnostic yield. Congored–stained tissue sections were retrospectively and prospectively examined by light microscopy (LM)with and without polarizer and by FM using the Texas red filter and results by each method compared. Congo red–stained amyloid recognized by LM was unequivocally and easily identified by FM in each of 48 cases. In 22 of them, FM either confirmed the presence of a small amount of amyloid or lead to adefinitive diagnosis, which was otherwise missed. Eight cases with Congo red–negative by LM were also negative by FM. In 8 cases with a false-positive Congo red stain, FM still detected the signal in 5, but it was absent in 3 cases.In conclusion, Congo red fluorescence improves the diagnostic yield of LMfor both positive and negative cases.

 

摘要:在偏振光下刚果红染色苹果绿双折射是用于检测淀粉样蛋白的最常用的方法;然而,它有局限性。本研究的目的是评估是否通过荧光显微镜(FM)的刚果红染色的检查能够显著提高诊断率。刚果红染色的组织切片通过有无偏光镜的光学显微镜(LM)与德州仪器红色滤光片的荧光显微镜(FM)同时比较每种方法的结果,进行了回顾性和前瞻性的研究。被LM识别的刚果红染色的淀粉样蛋白通过48例中每一个病例的 FM被明确地和容易地识别出。其中22例,FM要么证实少量淀粉样蛋白的存在,要么导致明确的诊断,其它的错过了。8LM检测刚果红为阴性的,FM检测也为阴性。在8例刚果红染色为假阳性,FM仍在5例中检测到信号,但另外3例中未检测到。总之,刚果红荧光改善了 LM对阳性和阴性案例的诊断率。