文献简介

出版社:Int Arch Allergy Immunol

作  者:Catharina Johansson a Niklas Ahlborg c, d Anna Andersson a Lena Lundeberg b Maria A. Karlsson b Annika Scheynius a Maria Tengvall Linder a

编  号:Catharina Johansson a Niklas Ahlborg c, d Anna Andersson a Lena Lundeberg b Maria A. Karlsson b Annika Scheynius a Maria Tengvall Linder a

关键字:Antigen-specific IgE ,Atopic eczema ,Atopic dermatitis ,Atopy patch test,ELISpot,Interleukin-4,Interleukin-5 ,Malassezia

年  份:2009   点击量:1052

文献摘要

Background:

Atopic eczema is a chronic inflammatory skin disease in which several subgroups of cases can be identified. Atopy patch testing (APT) reveals allergen sensitization also in atopic eczema patients devoid of detectable allergen- specific IgE, suggesting the importance of factors other than IgE in the reaction. Here we investigate the relationship between APT reactions and allergen-specific peripheral IgE and T cell reactivity in atopic eczema patients.

背景
过敏性湿疹是一种慢性炎症性皮肤病,它是几种能被鉴别的疾病亚群中的其中之一。斑贴试验(APT)揭示了过敏原致敏作用在过敏性湿疹患者体内也缺乏可检测的过敏原特异性IgE,提出在反应中除IgE之外的重要因素。这里我们研究APT反应和过敏原特异性外周IgE以及T细胞反应在过敏性湿疹患者中的关系

Methods:

Adult patients with atopic eczema (n = 64) and healthy controls (n = 24) were analyzed for reactivity to Malassezia sympodialis extract by APT, measurement of specific plasma IgE and in vitro determination of the frequency of allergen-reactive peripheral blood mononuclear cells producing interleukin- 4 and interleukin-5 using the ELISpot method.

方法

通过APT分析过敏性湿疹成人患者组(n=64)和健康对照组(n=24)对合轴马拉色菌提取物的反应,测量特异性血清IgE以及采用酶联免疫斑点检测法体外测定活性过敏原外周血单个核细胞产生白介素- 4和白介素-5的频率。

Results:

When combining the results of the APT, IgE measurements and the ELISpot analyses, reactivity to M. sympodialis was found in a majority of the atopic eczema patients (69%),whereas the healthy controls were negative throughout. T cell reactivity to M. sympodialis , manifested by production of both interleukins 4 and 5, was highly predictive for a positive APT reaction and displayed a strongly positive correlation with the APT score. In contrast, the allergen-specific IgE levels did not predict the APT outcome, and no correlation could be found between the IgE levels and the APT score.

结果

结合APTIgE测量值和ELISpot分析结果,我们发现大部分过敏性湿疹患者(69%)对合轴马拉色菌有反应,而健康对照组则(对合轴马拉色菌)全都无反应。T细胞对合轴马拉色菌有反应时表现为产生白介素-4和白介素-5,高度预测为阳性APT反应并且与APT值呈现强烈的正相关。相反情况下, 特异性过敏原IgE水平则无法预测APT的结果,并且 IgE水平和APT值之间并未发现有任何关系。

Conclusion:

Peripheral allergen-specific T helper 2 cell-mediated reactivity appears to be required for a positive APT reaction to M. sympodialis . The diagnostic potential of measuring peripheral allergen-specific T cell responses should be considered in atopic eczema.

总结

外周过敏原特异性辅助性T细胞2Th2)细胞介导反应似乎要求对合轴马拉色菌呈阳性APT反应。因此,通过测量外周过敏原特异性T细胞应答诊断潜在可能性时,应考虑过敏性湿疹。